How to quantify the response of a chromatogram
Could anyone give me the calculation for quantifying the analyte in HPLC
Replies to this topic (4)
2024-09-14 17:17:37
The concentration of the analyte is calculated based on the ratio of their peak areas and the known concentration of the internal standard
Concentration of analyte = (Peak area of analyte / Peak area of standard) × Concentration of standard
2024-09-14 17:22:49
Thanku
2024-09-14 18:49:02
to quantify a peak we need a known standard. The case where we dont have standard , to quantify within the chromatography, based on the peak height to run time we can quantify.
2024-09-15 05:50:35
To quantify the response of these peaks, we typically measure their height or area.
Peak Height
Peak Area
Concentration of the analyte
Detector sensitivity
Column efficiency
Injection volume
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