How to develop a HPLC method
How to develop a method for Assay by HPLC in waters or Agilent
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Developing an assay method by High-Performance Liquid Chromatography (HPLC) for water samples on either Waters or Agilent systems involves a systematic approach. While the specific details will depend on the analyte(s) of interest and the complexity of the water matrix
1. Define the Objective and Requirements
2. Literature Review
3. Preliminary Considerations and System Setup
4. Sample Preparation
5. Chromatographic Method Development:
6. Method Optimization
7. Method Validation
8. Documentation
By following these steps, you can develop a robust and reliable HPLC assay method for your target analytes in water samples using either Waters or Agilent HPLC systems. Remember that method development is often an iterative process, and you may need to revisit certain steps as you gather more information.