Retention Time and Relative Retention Time
Description
RT: - Retention time
Definition: -
Retention time is the time taken by the solute to pass through the chromatography column.
It is the time taken by the sample (compound) to elute from the time of injection
The factors that influence the Retention time were
- The flow rate of Mobile phase
Increase in flow rate results in early elution of the analyte there by reducing the Retention time.
- The composition of Mobile phase
The change in Mobile phase composition when there two or more buffers to mix, the retention time of the analyte will be altered.
In regular, increase in Organic proportion in the Mobile phase co
